Molecular analysis: Mass spectrometry and surrounding techniques

Course name: Molecular analysis: Mass spectrometry and surrounding techniques

Course no.: 3603.12

ECTS: 7.5

Level: Master and PhD

Prerequisites: Solid background in knowledge of molecules and molecular cell biology, like: 3512 General and inorganic chemistry, 3513 Organic chemistry, 3514 Biochemistry, 3518 Cell biology, 3516 Genetics and molecular biology and 3517 Microbiology; or equivalent courses. Experience in laboratory work exceeding the mentioned courses is strongly recommended.

When: Mondays, Wednesdays and Fridays, starting at 17th September 2012 at 09.15. Around 75 h of organized lectures, exercizes and laboratory work. Examination 1st November 2012.

Application deadline: 20th August 2012.

Location: NVD

Teachers: Svein-Ole Mikalsen, Hóraldur Joensen and Ása Jacobsen.

Language: Mainly English or Norwegian (according to choice of participants).

Number of participants: At maximum 4.

Objective: To provide the students with knowledge of mass spectrometry and some surrounding techniques.

Topics: Components of the mass spectrometer; ionization techniques (MALDI, ESI, APCI); mass analyzers (TOF, quadrupole, ion trap, orbitrap); MS/MS (CID, ETD, different types of MS/MS scans); understanding and interpretation of the mass spectrum (stable isotopes, isotopic envelope, exact mass and fractional mass); proteomics; liquid chromatography; gas chromatography; other themes connected to laboratory work may depend on background of the students. 

Laboratory practices and demonstrations (may be modified according to background of students): Protein electrophoresis; immunoprecipitation; isolelectric focusing; MS and MS/MS of peptides; preparations and analysis/identification of proteins; MS and MS/MS of small-molecular compounds. Students may bring own samples, and we will try to analyze them. The samples should be ready for MS analysis (work-up of samples should have been done in home lab).

Evaluation: Compulsory essay (written in scientific style) on use of mass spectrometry in the field of the student (counting 35%) + four-hour written examination (counting 65%). NVD computer available for writing. Electronic calculator allowed.

Examination evaluated externally. Essay evaluated internally.

Grading system: Faroese grading system (numbers)

Textbooks and materials: Edmond de Hoffmann and Vincent Stroobant: Mass Spectrometry – Principles and Applications, 3^rd ed. Wiley 2007, ISBN 978-0-470-03311-1.

Ingvar Eidhammer, Kristian Flikka, Lennart Martens, Svein-Ole Mikalsen: Computational Methods for Mass Spectrometry Proteomics. Wiley 2007, ISBN 978-0-470-51297-5.

Hand-outs.

Suggested complementary textbook: Jurgen H Gross: Mass Spectrometry - A Textbook, 2nd ed. Springer 2011, ISBN 978-3-642-10709-2

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