Svein-Ole Mikalsen

Dr.philos. (=Ph.D.)

Professor in molecular biology

Department of Science and Technology

Faculty of Natural and Health Sciences

University of the Faroe Islands

Contact info:

Tel: +298 352 556

E-mail: sveinom(at)setur.fo

Education
1978-1982    University of Oslo, cand.mag. (=BSc)
1983-1985    University of Oslo, cand.scient. (=MSc)
1986-1992    University of Oslo, Dr.philos. (=PhD)

Employment
1983-1985    Master thesis:  “Mineral fibres and cell transformation. Effects of asbestos fibres and glass fibres in the Syrian hamster embryo cell transformation assay“. Performed at The Norwegian Radium Hospital Oslo, Dept. of Environmental and Occupational Cancer. Supervised by professor Tore Sanner and Dr. Edgar Rivedal.

1986-1991    Doctoral fellowship from the Norwegian Cancer Society. Dr.philos. thesis. “Cell transformation and intercellular communication. Testing of non-genotoxic carcinogens and mechanistic considerations with special reference to hepatic peroxisome proliferators”. Affiliation: The Norwegian Radium Hospital Oslo, Dept. of Environmental and Occupational Cancer. Supervised by professor Tore Sanner.

1992-1994    Postdoctoral fellowship from the Norwegian Research Council. Affiliation: The Norwegian Radium Hospital Oslo, Dept. of Environmental and Occupational Cancer.

1995-2009    Scientist at The Norwegian Radium Hospital Oslo, Dept. of Environmental and Occupational Cancer. The department and the hospital have changed their formal names and organization. The department is today called Department of Cancer Prevention. The hospital has fused with the other hospitals in the Oslo area, and is now formally named Clinic for Cancer Medicine, Surgery and Transplantation, Oslo University Hospital.

2009-present    Professor in molecular biology, Department of Science and Technology, Faculty of Natural and Health Sciences, University of the Faroe Islands.

Scientific publications in short
57 printed papers in international peer-reviewed journal listed in PubMed
1 textbook (within mass spectrometry and bioinformatics)
7 book chapters, scientific reports, etc.
Approx. 70 abstracts from scientific meetings and conferences
1 doctoral thesis
1 master thesis
40 sequences in GenBank

Books
1. Eidhammer I, Flikka K, Martens L, Mikalsen S-O. Computational methods for mass spectrometry proteomics. John Wiley & Sons, Ltd. 284 pp. 2007. ISBN 978-0-470-51297-5.

Papers in peer-reviewed international journals (inverse chronological order)
57. Johansen D, Cruciani V, Sundset R, Ytrehus K, Mikalsen S-O (2011) Ischemia induces closure of gap junctional channels and opening of hemichannels in heart-derived cells and tissue. Cell Physiol Biochem 28, 103-114.

56. Sundset R, Ytrehus K, Mikalsen S-O (2009) Connexin, connection, conductance: Towards understanding induction of arrhythmias? Heart Rhythm 6, 1639-1640.

55. Barsnes H, Mikalsen S-O, Eidhammer I (2008) Blind search for post-translational modifications and amino acid substitutions using peptide mass fingerprints from two proteases. BMC Res Notes 1, 130.

54. Barsnes H, Eidhammer I, Cruciani V, Mikalsen S-O (2008) Protease-dependent fractional mass and peptide properties. Eur J Mass Spectrom 14, 311-317.

53. Haugen MH, Flatmark K, Mikalsen S-O, Malandsmo GM (2008) The metastasis-associated protein S100A4 exists in several charged variants suggesting the presence of posttranslational modifications. BMC Cancer 8, 172.

52. Cruciani V, Mikalsen S-O (2007) Evolutionary selection pressure and family relationships among connexin genes. Biol Chem 388, 253-264. Additional supplementary materials, 118 pp.

51. Cruciani V, Mikalsen S-O (2006) The vertebrate connexin family. Cell Mol Life Sci 63, 1125-1140.

50. Barsnes H, Mikalsen S-O, Eidhammer I (2006) MassSorter: a tool for administrating and analyzing data from mass spectrometry experiments on proteins with known amino acid sequence. BMC Bioinformatics 7, 42.

49. Cruciani V, Heintz K-M, Husøy T, Hovig E, Warren DJ, Mikalsen S-O (2004) The detection of hamster connexins: a comparison of expression profiles with wild-type mouse and the cancer-prone Min mouse. Cell Commun Adhes 11, 155-171.

48. Lindstad RI, Sylte I, Mikalsen S-O, Seglen PO, Berg E, Winberg J-O (2005) Pancreatic trypsin activates human promatrix metalloproteinase-2. J Mol Biol 350, 682-698.

47. Husøy T, Knutsen HK, Cruciani V, Ølstørn HB, Mikalsen S-O, Løberg EM, Alexander J (2005) Connexin43 is overexpressed in Apc^Min/+-mice adenomas and co-localises with COX-2 in myofibroblasts. Int J Cancer 116, 351-358.

46. Sundset R, Cooper M, Mikalsen S-O, Ytrehus K (2004) Ischemic preconditioning protects against gap junctional uncoupling in cardiac myofibroblasts. Cell Commun Adhes 11, 51-66.

45. Cruciani V, Mikalsen S-O (2005)  The connexin gene family in mammals. Biol Chem 386, 325-332. Additional supplementary materials, 36 pp.

44. Jørgensen K, Skrede M, Cruciani V, Mikalsen S-O, Slipicevic A, Flørenes VA (2005) Phorbol ester phorbol-12-myristate-13-acetate (PMA) promotes anchorage-independent growth and survival of melanomas through MEK-independent activation of ERK1/2. Biochem Biophys Res Commun 329, 266-274.

43. Cruciani V, Mikalsen S-O (2005) Ilimaquinone inhibits gap junctional communication in a connexin isotype-specific manner. Exp Cell Res 304, 136-148.

42. Husøy T, Ølstørn HB, Knutsen HK, Løberg EM, Cruciani V, Mikalsen S-O, Goverud IL, Alexander J (2004) Truncated mouse adenomatous polyposis coli reduces expression of connexin32 and increases secretion of matrilysin from Paneth cells. Eur J Cancer 40, 1599-1603.

41. Flatmark K, Mælandsmo GM, Mikalsen S-O, Nustad K, Varaas T, Rasmussen H, Meling GI, Fodstad Ø, Paus E (2004) Immunofluorimetric assay for the metastasis-related protein S100A4: Release of S100A4 from normal blood cells prohibits the use of S100A4 as a tumor marker in plasma and serum. Tumor Biol 25, 31-40.

40. Prasmickaite L, Høgset A, Olsen VM, Kaalhus O, Mikalsen S-O, Berg K (2004) Photochemically enhanced gene transfection increases the cytotoxicity of the herpes simplex virus thymidine kinase gene combined with ganciclovir. Cancer Gene Therapy 11, 514-523.

39. Leithe E, Cruciani V, Sanner T, Mikalsen S-O, Rivedal E (2003) Recovery of gap junctional intercellular communication after phorbol ester treatment requires proteasomal degradation of protein kinase C. Carcinogenesis 24, 1239-1245.

38. Cruciani V, Leithe E, Mikalsen S-O (2003) Ilimaquinone inhibits gap-junctional communication prior to Golgi fragmentation and block in protein transport. Exp Cell Res 287, 130-142.

37. Flatmark K, Pedersen KB, Nesland JM, Rasmussen H, Aamodt G, Mikalsen S-O, Bjørnland K, Fodstad Ø, Mælandsmo GM (2003) Nuclear localization of the metastasis-related protein S100A4 correlates with tumour stage in colorectal cancer. J Pathol. 200, 589-595.

36. Husøy T, Cruciani V, Knutsen HK, Mikalsen S-O, Ølstørn HB, Alexander J. (2003) Cells heterozygous for the Apc^Min mutation have decreased gap junctional intercellular communication and connexin43 level, and reduced microtubule polymerization. Carcinogenesis 24, 643-650.

35. Cruciani V, Mikalsen S-O (2002) Connexins, gap junctional intercellular communication and kinases. Biol Cell 94, 433-443.

34. Cruciani V, Mikalsen S-O (2002) Mechanisms involved in responses to the peroxisome proliferator WY-14,643 on gap junctional intercellular communication in V79 hamster fibroblasts. Toxicol Appl Pharmacol 182, 66-75.

33. Cruciani V, Husøy T, Mikalsen S-O (2001) Pharmacological evidence for system-dependent involvement of protein kinase C isoenzymes in phorbol ester-suppressed gap junctional communication. Exp Cell Res 268, 150-161.

32. Husøy T, Cruciani V, Sanner T, Mikalsen S-O (2001). Phosphorylation of connexin43 and inhibition of gap junctional communication in 12-O-tetradecanoylphorbol-13-acetate exposed R6 fibroblasts: minor role of protein kinase CbetaI and mu. Carcinogenesis 22, 221-231.

31. Dahle J, Mikalsen S-O, Rivedal E, Steen HB (2000) Gap junctional intercellular communication is not a major mediator in the bystander effect in photodynamic treatment of MDCK II cells. Radiat Res 154, 331-341.

30. Rivedal E, Mikalsen S-O, Sanner T (2000) Morphological transformation and effect on gap junctional intercellular communication in Syrian hamster embryo cells as screening tests for carcinogens devoid of mutagenic activity. Toxicol in Vitro 14, 185-192.

29. Cruciani V, Kaalhus O, Mikalsen S-O (1999). Phosphatases involved in modulation of gap junctional intercellular communication and dephosphorylation of connexin43 in hamster fibroblasts: 2B or not 2B? Exp Cell Res 252, 449-463.

28. Cruciani V, Mikalsen S-O (1999) Stimulated phosphorylation of intracellular connexin43. Exp Cell Res 251, 285-298.

27. Mikalsen S-O, Kaalhus O (1998) Properties of pervanadate and permolybdate: Connexin43, phosphatase inhibition, phosphatase inhibition and thiol reactivity as model systems. J Biol Chem 273, 10036-10045.

26. Cruciani V, Mikalsen S-O, Vasseur P, Sanner T (1997) Effects of peroxisome proliferators and 12-O-tetradecanoyl phorbol-13-acetate on intercellular communication and connexin43 in two hamster fibroblast systems. Int J Cancer 73, 240-248.

25. Mikalsen S-O, Kaalhus O (1997) A characterization of permolybdate and its effect on gap junctional intercellular communication, cellular tyrosine phosphorylation and phosphorylation status of the gap junction protein, connexin43. Biochim Biophys Acta 1356, 207-220.

24. Mikalsen S-O, Husøy T, Vikhamar G, Sanner T (1997) Induction of tyrosine phosphorylation in the gap junction protein, connexin43. FEBS Lett 401, 271-275.

23. Mikalsen S-O, Kaalhus O (1996) A characterization of pervanadate, an inducer of cellular tyrosinephosphorylation and inhibitor of gap junctional intercellular communication. Biochim Biophys Acta, 1290, 308-318.

22. Husøy T, Mikalsen S-O, Sanner T (1995) Gap junctional intercellular communication and modulators of kinases and phosphatases. ATLA 23, 480-484.

21. Mikalsen S-O, Modalsli KR (1994) A simple fluorescence method for the study of the internalization of particles in cultured cells: application to asbestos and glass fibres. Toxicology in Vitro 8, 243-250.

20. Bånrud H, Mikalsen S-O, Berg K, Moan J (1994) Effects of ultraviolet radiation on intercellular communication in V79 Chinese hamster fibroblasts. Carcinogenesis 15, 233-239.

19. Mikalsen S-O, Sanner T (1994) Increased gap junctional intercellular communication in Syrian hamster embryo cells treated with oxidative agents. Carcinogenesis 15, 381-387.

18. Husøy T, Mikalsen S-O, Sanner T (1993) Phosphatase inhibitors, gap junctional intercellular communication and [¹²⁵I]-EGF binding in hamster fibroblasts. Carcinogenesis 14, 2257-2265.

17. Mikalsen S-O (1993) Heterologous gap junctional intercellular communication in normal and morphologically transformed colonies of Syrian hamster embryo cells. Carcinogenesis 14, 2085-2090.

16. Modalsli KR, Mikalsen S-O, Bukholm G, Degre M (1993) Microinjection of HEp-2 cells with coxsackie B1 virus RNA enhances invasiveness of Shigella flexneri only after prestimulation with UV-inactivated virus. APMIS 101, 602-606.

15. Mikalsen S-O, Sanner T (1993) Intercellular communication in colonies of Syrian hamster embryo cells and the susceptibility for morphological transformation. Carcinogenesis 14, 251-257.

14. Husøy T, Mikalsen S-O, Sanner T (1993) Effects of five phorbol esters on gap junctional intercellular communication, morphological transformation and epidermal growth factor binding in Syrian hamster embryo cells. Carcinogenesis 14, 73-77.

13. Mikalsen S-O, Rivedal E, Sanner T (1992) Heavy metal ions, cytotoxicity and gap junctional intercellular communication in Syrian hamster embryo cells. ATLA 20, 213-217.

12. Modalsli KR, Bukholm G, Mikalsen S-O, Degre M (1992) Coxsackie B-1 virus-induced changes in cell membrane-associated functions are not responsible for altered sensitivity to bacterial invasiveness. Arch Virology 124, 321-332.

11. Rivedal E, Mikalsen S-O, Roseng E, Sanner T, Eide I (1992) Effects of hydrocarbons on transformation and inter-cellular communication in Syrian hamster embryo cells. Pharmacol Toxicol 71, 57-61.

10. Modalsli KR, Bukholm G, Mikalsen S-O, Degre M (1992) Correlation between coxsackie B1 virus replication and enhanced invasiveness of Shigella flexneri. APMIS 100, 237-245.

9. Modalsli KR, Bukholm G, Mikalsen S-O, Degre M (1991) Microinjected coxsackie B1 virus does not replicate in HEp-2 cells. Virology, 185, 888-890.

8. Mikalsen S-O, Kaalhus O, Reith A, Sanner T (1990) Role of catalase and oxidative stress hepatic peroxisome proliferator-induced  morphological transformation of Syrian hamster embryo cells. Int J Cancer 46, 950-957.

7. Mikalsen S-O, Ruyter B, Sanner T (1990) Effects of hepatic peroxisome proliferators and 12-O-tetradecanoyl phorbol-13-acetate on catalase and other enzyme activities of embryonic cells in vitro. Biochem Pharmacol, 39, 527-535.

6. Mikalsen S-O, Holen I, Sanner T (1990) Morphological transformation and catalase activity of Syrian hamster embryo cells treated with hepatic peroxisome proliferators, TPA and nickel sulphate. Cell Biol Toxicol, 6, 1-13.

5. Mikalsen S-O (1990) Effects of heavy metal ions on intercellular communication in Syrian hamster embryo cells. Carcinogenesis 11, 1621-1626.

4. Rivedal E, Mikalsen S-O, Sanner T (1990) The non-phorbol ester tumor promoter okadaic acid does not promote morphological transformation or inhibit junctional communication in hamster embryo cells. Biochem Biophys Res Commun, 167, 1302-1308.

3. Mikalsen S-O (1990) Morphological transformation of Syrian hamster embryo cells by aminobenzyl alcohols and nitrobenzyl alcohols is correlated with intercellular communication. Cancer Letters 53, 203-208.

2. Holen I, Mikalsen S-O, Sanner T (1990) Effects of dinitrotoluenes on morphological cell transformation and intercellular communication in Syrian hamster embryo cells. J Toxicol Environ Health 29, 89-98.

1. Mikalsen S-O, Rivedal E, Sanner T (1988) Morphological transformation of Syrian hamster embryo cells induced by mineral fibres and the alleged enhancement by benzo(a)pyrene. Carcinogenesis 9, 891-899.

Book chapters, etc.
6. Eidhammer I, Barsnes H, Mikalsen S-O (2008) MassSorter: peptide mass fingerprinting data analysis. In: Thompson JD, Schaeffer-Reiss C, Ueffing M (eds) Methods in Molecular Biology Vol. 484: Functional Proteomics, Humana Press, Inc., Totowa, NJ, pp. 345-359.

5. Rivedal E, Mikalsen S-O, Roseng LE, Yamasaki H, Sanner T (1995) Cell communication and morphological transformation in hamster embryo fibroblasts. In: Kanno Y et al: Progress in Cell Research Vol. 4, Elsevier Sci. Publ., Amsterdam, pp. 137-140.

4. Mikalsen S-O, Husøy T, Sanner T (1995) Modulation of gap junctional intercellular communication by phosphorylation. Effects of growth factors, kinase activators and phosphatase inhibitors. In: McClain RM, Slaga TJ, LeBoeuf R and Pitot H (eds) Progress in Clinical and Biological Research, Vol. 391: Growth factors and Tumor Promotion: Implications for Risk Assessment. Wiley-Liss, New York, pp. 425-438.

3. Mikalsen S-O (1991) Asbestos-induced morphological transformation of cells in culture. In: Sluyser M (ed) Asbestos-Related Cancer. Ellis Horwood, New York, pp. 107-123.

2. Sanner T, Mikalsen S-O, Rivedal E (1991) Hepatic peroxisome proliferators induce morphological transformation of Syrian hamster embryo cells, but not peroxisomal ß-oxidation. In Butterworth BE, Slaga TJ, Farland W and McClain M (eds.) Progress in Clinical and Biological Research, Vol. 369: Chemically Induced Cell Proliferation: Implications for Risk Assessment. Wiley-Liss, New York, pp.77-89.

1. Rivedal E, Haddeland U, Mikalsen S-O, Roseng LE, Sanner T (1990) Modulation of induction of morphological transformation. In: Proceedings of the Syrian Hamster Embryo Cell Transformation Workshop. Wintergreen, Virginia, USA, pp. 189-200.

Reports, etc.
1. Dybing E, Mikalsen S-O, Huttunen KJ, Sanner T (1995) Peroxisome proliferation, genotoxicity, and carcinogenicity. IARC Technical Report Series 24, 55-85.

Master and doctoral theses
1. Mikalsen S-O (1985) Mineral fibres and cell transformation. Effects of asbestos fibres and glass fibres in the Syrian hamster embryo cell transformation assay. Master thesis for the degree of cand. scient. (=MSc), Biological Institute, University of Oslo (in Norwegian).

2. Mikalsen S-O (1992) Cell transformation and intercellular communication. Testing of "non-genotoxic" carcinogens and mechanistic considerations with special reference to hepatic peroxisome proliferators. Thesis for the degree of dr. philos (=PhD), University of Oslo.